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The antibody is from purified rabbit serum by affinity purification by means of sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
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Overexpression of the gene has long been noticed in many tumors, which results in chromosome instability, and thus could lead to tumorigenesis. This protein was located to associate with, and be involved with, the phosphorylation of NPAT protein (nuclear protein mapped to your ATM locus), which participates in mobile-cycle controlled histone gene expression and performs a important purpose in selling cell-cycle progression inside the absence of pRB. Two alternatively spliced transcript variants of this gene, which encode distinctive isoforms, have been explained. Two supplemental splice variants were claimed but in-depth nucleotide sequence data isn't still obtainable.
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Blocking peptides are peptides that bind specifically on the concentrate on antibody and block antibody binding. These peptide generally is made up of the epitope recognized through the antibody.
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This information outlines additional possible optimization of cellular and immunoassay parameters to make sure the very best effects are...
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This manual outlines even more attainable optimization of mobile and immunoassay parameters to ensure more info the absolute best benefits are...
Antibodies certain to the blocking peptide not bind on the epitope within the goal protein. By comparing the staining in the blocked antibody versus the antibody alone, you can see which staining is unique.
Focus on Info Cyclin E belongs into the really conserved cyclin spouse and children, whose customers are characterized by a extraordinary periodicity in protein abundance from the cell cycle. Cyclins operate as regulators of CDK kinases. Unique cyclins show unique expression and degradation patterns which lead towards the temporal coordination of each and every mitotic function. Cyclin E forms a fancy with and capabilities for a regulatory subunit of CDK2, whose action is required for cell cycle G1/S changeover. This protein accumulates in the G1-S period boundary and is degraded as cells progress as a result of S stage.
The measurement of protein phosphorylation is a useful tool for measuring the modulation of receptor activation by the two antibodies...
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